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monoclonal rabbit α stat1  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc monoclonal rabbit α stat1
    Nuclear translocation of STAT family members during E. chaffeensis infection. ( A ) Uninfected or E. chaffeensis -infected THP-1 cells at 72 hpi (MOI 10) were probed for <t>STAT1–6</t> (green) and E. chaffeensis marker Dsb (red) by immunofluorescent microscopy. 4´,6-Diamidino-2-phenylindole (DAPI) (blue) was used to stain nuclei. Scale bar = 20 µm. ( B ) Nuclear regions of interest (ROIs) were defined by DAPI signal, and mean nuclear STAT3 intensity per cell was quantified using ImageJ. Statistical significance was determined using two-tailed Student’s t -test; * P < 0.05, ** P < 0.01. Data represent the mean ± SD of three independent biological replicates ( n = 3), and representative images are shown.
    Monoclonal Rabbit α Stat1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 985 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/monoclonal+rabbit+%CE%B1+stat1/Stat1+Rabbit+mAb/pmc12604489-158-8-12
    Average 97 stars, based on 985 article reviews
    monoclonal rabbit α stat1 - by Bioz Stars, 2026-11
    97/100 stars

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    1) Product Images from "TRP75-mediated STAT3 activation promotes anti-apoptotic signaling and Ehrlichia chaffeensis infection"

    Article Title: TRP75-mediated STAT3 activation promotes anti-apoptotic signaling and Ehrlichia chaffeensis infection

    Journal: Infection and Immunity

    doi: 10.1128/iai.00459-25

    Nuclear translocation of STAT family members during E. chaffeensis infection. ( A ) Uninfected or E. chaffeensis -infected THP-1 cells at 72 hpi (MOI 10) were probed for STAT1–6 (green) and E. chaffeensis marker Dsb (red) by immunofluorescent microscopy. 4´,6-Diamidino-2-phenylindole (DAPI) (blue) was used to stain nuclei. Scale bar = 20 µm. ( B ) Nuclear regions of interest (ROIs) were defined by DAPI signal, and mean nuclear STAT3 intensity per cell was quantified using ImageJ. Statistical significance was determined using two-tailed Student’s t -test; * P < 0.05, ** P < 0.01. Data represent the mean ± SD of three independent biological replicates ( n = 3), and representative images are shown.
    Figure Legend Snippet: Nuclear translocation of STAT family members during E. chaffeensis infection. ( A ) Uninfected or E. chaffeensis -infected THP-1 cells at 72 hpi (MOI 10) were probed for STAT1–6 (green) and E. chaffeensis marker Dsb (red) by immunofluorescent microscopy. 4´,6-Diamidino-2-phenylindole (DAPI) (blue) was used to stain nuclei. Scale bar = 20 µm. ( B ) Nuclear regions of interest (ROIs) were defined by DAPI signal, and mean nuclear STAT3 intensity per cell was quantified using ImageJ. Statistical significance was determined using two-tailed Student’s t -test; * P < 0.05, ** P < 0.01. Data represent the mean ± SD of three independent biological replicates ( n = 3), and representative images are shown.

    Techniques Used: Translocation Assay, Infection, Marker, Microscopy, Staining, Two Tailed Test

    Related Articles

    Cell-Signaling:

    Article Title: TRP75-mediated STAT3 activation promotes anti-apoptotic signaling and Ehrlichia chaffeensis infection
    Article Snippet: .. The primary antibodies utilized in this research include monoclonal rabbit α-STAT1 (14994S; Cell Signaling Technology), monoclonal rabbit α-STAT2 (72604S; Cell Signaling Technology), rabbit α-STAT3 (12640S; Cell Signaling Technology), monoclonal rabbit α-STAT4 (2653S; Cell Signaling Technology), monoclonal rabbit α-STAT5 (94205S; Cell Signaling Technology), monoclonal rabbit α-STAT6 (5397S; Cell Signaling Technology), monoclonal rabbit α-phospho-STAT3 Y705 (9145S; Cell Signaling Technology), monoclonal rabbit α-MCL-1 (94296S; Cell Signaling Technology), monoclonal rabbit α-caspase 3 (9662S; Cell Signaling Technology), monoclonal mouse α-caspase 7 (9494S; Cell Signaling Technology), monoclonal mouse α-GAPDH (sc-47724; Santa Cruz Biotechnology), monoclonal mouse α-Tubulin (sc-5286; Santa Cruz Biotechnology), monoclonal mouse α-Vinculin (sc-73614; Santa Cruz Biotechnology), monoclonal mouse α-GFP (sc-9996; Santa Cruz Biotechnology), rabbit α-disulfide bond formation protein (Dsb) , and rabbit α-TRP75 ( ). .. Secondary antibodies included horseradish peroxidase-conjugated goat anti-rabbit IgG (5450-0010; SeraCare) and anti-mouse IgG (5220-0341; SeraCare), used for immunoblotting, and Alexa Fluor Plus 488- or 594-conjugated goat anti-rabbit or anti-mouse IgG (A32731, A32723, A32740, A32742; Invitrogen), used for immunofluorescence microscopy.



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    monoclonal rabbit α stat1  (Cell Signaling Technology Inc)
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    Cell Signaling Technology Inc monoclonal rabbit α stat1
    Nuclear translocation of STAT family members during E. chaffeensis infection. ( A ) Uninfected or E. chaffeensis -infected THP-1 cells at 72 hpi (MOI 10) were probed for <t>STAT1–6</t> (green) and E. chaffeensis marker Dsb (red) by immunofluorescent microscopy. 4´,6-Diamidino-2-phenylindole (DAPI) (blue) was used to stain nuclei. Scale bar = 20 µm. ( B ) Nuclear regions of interest (ROIs) were defined by DAPI signal, and mean nuclear STAT3 intensity per cell was quantified using ImageJ. Statistical significance was determined using two-tailed Student’s t -test; * P < 0.05, ** P < 0.01. Data represent the mean ± SD of three independent biological replicates ( n = 3), and representative images are shown.
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    https://www.bioz.com/product/monoclonal+rabbit+%CE%B1+stat1/Stat1+Rabbit+mAb/pmc12604489-158-8-12
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    Nuclear translocation of STAT family members during E. chaffeensis infection. ( A ) Uninfected or E. chaffeensis -infected THP-1 cells at 72 hpi (MOI 10) were probed for <t>STAT1–6</t> (green) and E. chaffeensis marker Dsb (red) by immunofluorescent microscopy. 4´,6-Diamidino-2-phenylindole (DAPI) (blue) was used to stain nuclei. Scale bar = 20 µm. ( B ) Nuclear regions of interest (ROIs) were defined by DAPI signal, and mean nuclear STAT3 intensity per cell was quantified using ImageJ. Statistical significance was determined using two-tailed Student’s t -test; * P < 0.05, ** P < 0.01. Data represent the mean ± SD of three independent biological replicates ( n = 3), and representative images are shown.
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    Image Search Results


    Nuclear translocation of STAT family members during E. chaffeensis infection. ( A ) Uninfected or E. chaffeensis -infected THP-1 cells at 72 hpi (MOI 10) were probed for STAT1–6 (green) and E. chaffeensis marker Dsb (red) by immunofluorescent microscopy. 4´,6-Diamidino-2-phenylindole (DAPI) (blue) was used to stain nuclei. Scale bar = 20 µm. ( B ) Nuclear regions of interest (ROIs) were defined by DAPI signal, and mean nuclear STAT3 intensity per cell was quantified using ImageJ. Statistical significance was determined using two-tailed Student’s t -test; * P < 0.05, ** P < 0.01. Data represent the mean ± SD of three independent biological replicates ( n = 3), and representative images are shown.

    Journal: Infection and Immunity

    Article Title: TRP75-mediated STAT3 activation promotes anti-apoptotic signaling and Ehrlichia chaffeensis infection

    doi: 10.1128/iai.00459-25

    Figure Lengend Snippet: Nuclear translocation of STAT family members during E. chaffeensis infection. ( A ) Uninfected or E. chaffeensis -infected THP-1 cells at 72 hpi (MOI 10) were probed for STAT1–6 (green) and E. chaffeensis marker Dsb (red) by immunofluorescent microscopy. 4´,6-Diamidino-2-phenylindole (DAPI) (blue) was used to stain nuclei. Scale bar = 20 µm. ( B ) Nuclear regions of interest (ROIs) were defined by DAPI signal, and mean nuclear STAT3 intensity per cell was quantified using ImageJ. Statistical significance was determined using two-tailed Student’s t -test; * P < 0.05, ** P < 0.01. Data represent the mean ± SD of three independent biological replicates ( n = 3), and representative images are shown.

    Article Snippet: The primary antibodies utilized in this research include monoclonal rabbit α-STAT1 (14994S; Cell Signaling Technology), monoclonal rabbit α-STAT2 (72604S; Cell Signaling Technology), rabbit α-STAT3 (12640S; Cell Signaling Technology), monoclonal rabbit α-STAT4 (2653S; Cell Signaling Technology), monoclonal rabbit α-STAT5 (94205S; Cell Signaling Technology), monoclonal rabbit α-STAT6 (5397S; Cell Signaling Technology), monoclonal rabbit α-phospho-STAT3 Y705 (9145S; Cell Signaling Technology), monoclonal rabbit α-MCL-1 (94296S; Cell Signaling Technology), monoclonal rabbit α-caspase 3 (9662S; Cell Signaling Technology), monoclonal mouse α-caspase 7 (9494S; Cell Signaling Technology), monoclonal mouse α-GAPDH (sc-47724; Santa Cruz Biotechnology), monoclonal mouse α-Tubulin (sc-5286; Santa Cruz Biotechnology), monoclonal mouse α-Vinculin (sc-73614; Santa Cruz Biotechnology), monoclonal mouse α-GFP (sc-9996; Santa Cruz Biotechnology), rabbit α-disulfide bond formation protein (Dsb) , and rabbit α-TRP75 ( ).

    Techniques: Translocation Assay, Infection, Marker, Microscopy, Staining, Two Tailed Test